The Gadsden flag, featuring a rattlesnake and the word "Don't Tread on Me

https://travismqdt216.zenbloomer.com/posts/collecting-civil-war-flags-a-guide-for-enthusiasts-and-historians-2

The Gadsden flag, providing a rattlesnake and the phrase "Don't Tread on Me," become adopted through some Confederate contraptions as a image of resistance in opposition to perceived oppression.